Advertisement

BS-512-03 HIGH-RESOLUTION STRUCTURE-FUNCTION MAPPING OF INTACT HEARTS REVEALS ALTERED SYMPATHETIC CONTROL OF INFARCT BORDER ZONES

      Background

      Intramyocardial sympathetic nerve remodeling after myocardial infarction (MI) contributes to adverse outcomes such as sudden arrhythmic death, yet the underlying structural mechanisms are poorly understood. This is partly due to challenges in directly correlating high-resolution structural and electrical data from the same heart.

      Objective

      We sought to examine microstructural changes on the intact post-MI heart and to directly link these changes with electrical dysfunction.

      Methods

      We developed a high-resolution pipeline for anatomically precise alignment of electrical maps with structural myofiber and nerve-fiber maps created by customized computer vision algorithms.

      Results

      Using this integrative approach in a mouse model, we identified distinct structure-function correlates to objectively delineate the infarct border zone, a known source of post-MI arrhythmias. During tyramine-induced sympathetic nerve activation, we demonstrated regional patterns of altered electrical conduction aligned directly with altered neuroeffector junction distribution, pointing to potential neural substrates for cardiac arrhythmia.